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SRX4221220: GSM3192002: RAID_SetA; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 15.4M spots, 1.1G bases, 454.2Mb downloads

Submitted by: NCBI (GEO)
Study: Comparison of single-cell transcriptomics quality between unfixed cells and cells that were fixed and mock stained according to the RAID procedure
PRJNA476320 • SRP150624 • All experiments • All runs
show Abstracthide Abstract
Cell fixation, permeabilization and antibody staining of could have adverse effects on the quality of single cell transcriptomics data. To assess the effects of the RAID procedure, which includes such steps, we performed a direct comparison of single cell transcriptomics by CELseq2 using unfixed and RAID-processed cells. Quality measures (gene complexity, gene detection rate, average gene expression) were performed using 40000 samples UMI counts per cell. Overall design: Single cells were sorted in 96, wells plates. Per condition (unfixed or RAID) three sets (A,B,C) of 48 cells were processed with the CELseq2 protocol.
Sample: RAID_SetA
SAMN09429725 • SRS3421895 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Reverse Transcription from detergent and heat based lysates, according to the SORT-seq/CELseq2 protocol CELseq2 library prep
Experiment attributes:
GEO Accession: GSM3192002
Links:
Runs: 1 run, 15.4M spots, 1.1G bases, 454.2Mb
Run# of Spots# of BasesSizePublished
SRR734782915,402,2351.1G454.2Mb2019-02-15

ID:
5708207

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